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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Cytotherapy. 2010 Oct;12(6):807–817. doi: 10.3109/14653249.2010.491821

Figure 6.

Figure 6

p38MAPK inhibitor-treated hESC differentiate into all three germ layers in vitro. (A) Immunofluorescence staining of SB203580-treated (p38i) and untreated (Ctl) hEB on day 30 of differentiation demonstrated expression of markers of all three embryonic germ layers, namely nestin and βIII tubulin (ectoderm), α-fetoprotein (AFP; endoderm) and smooth muscle actin (SMA) and cTnT (mesoderm). Bar 10 μm. (B) hEB from SB203580-treated (p38i) and untreated (Ctl) cultures collected prior to treatment, at day 4, and at day 14 were assayed for germ layer-specific gene expression by qPCR. Expression was normalized to GAPDH and calculated relative to proliferating, undifferentiated hESC. There was no significant difference between expression levels of βIII tubulin, SMA or α-fetoprotein in treated and untreated cultures. Data shown are mean percentage ± SEM (n = 3).