Table 2.

Table 2-1. IHC and direct sequencing details (N=70)
	Sample No.	IHC	H score max	Direct sequencing	Nucleotide number and sequence	Amino-acid change
Exon 19 deletions	56	Del	400	Del	2236-2250del15	del E746–A750
	49	Del	280	Del	2235-2249del15	del E746–A750
	41	Del	230	Del	2235-2249del15	del E746–A750
	46	Del	205	Del	2235-2249del15	del E746–A750
	69	Del	190	Del	2235-2249del15	del E746–A750
	16	Del	110	Del	2235-2249del15	del E746–A750
	3	Del	100	Del	2235-2249del15	del E746–A750
	43	Del	100	Del	2235-2249del15	del E746–A750
	61	Del	100	Del	2235-2249del15	del E746–A750
	27	Wt	5	Del	2235-2249del15	del E746–A750
	4	Wt	0	Del	2236-2250del15	del E746–A750
	42	Wt	0	Del	2239-2253del15*	delL747-T751
	50	Wt	0	Del	2239-2253del15*	delL747-T751
	67	Wt	0	Del	2239-2253del15*	delL747-T751
	24	Wt	0	Del	2240-2257del18*	del L747-P753insS
	40	Wt	0	Del	2240-2257del18*	del L747-P753insS
	55	Wt	0	Del	2240-2257del18*	del L747-P753insS
	18	Wt	0	Del	2253-2276del24*	S752-I759
Exon 21 mutations	32	L858R	370	L858R or Wt	2573 T>K	L858R or Wt
	68	L858R	360	L858R	2573 T>G	L858R
	1	L858R	240	L858R	2573 T>G	L858R
	66	L858R	240	L858R	2573 T>G	L858R
	51	L858R	230	L858R or Wt	2573 T>K	L858R or Wt
	31	L858R	200	L858R or Wt	2573 T>K	L858R or Wt
	12	L858R	100	L858R or Wt	2573 T>K	L858R or Wt
	21	L858R	80	L858R	2573 T>G	L858R
	60	L858R	20	L858R	2573 T>G	L858R
	65	L858R	215	Wt
	58	L858R	180	Wt
	62	Wt	0	L858R	2573 T>G	L858R
	5	Wt	0	L858R or Wt	2573 T>K	L858R or Wt
	33	Wt	0	L858R or Wt	2573 T>K	L858R or Wt

Table 2-2. Immunoreactivity of E746-A750 specific and L858R specific antibodies
	Sample No.	IHC	Amino acid change	H score		Staining area (%) per intensity
				max	mean	0	1+	2+	3+	4+
E746-A750 specific antibody	56	Del	del E746–A750	400	400	0	0	0	0	100
	49	Del	del E746–A750	280	225	0	4	68	28	0
	41	Del	del E746–A750	230	116.7	50	0	33	17	0
	46	Del	del E746–A750	205	198.3	2	0	96	2	0
	69	Del	del E746–A750	190	163.3	0	67	13	10	10
	16	Del	del E746–A750	110	105	0	95	5	0	0
	3	Del	del E746–A750	100	36.7	65	33	2	0	0
	43	Del	del E746–A750	100	33.3	67	33	0	0	0
	61	Del	del E746–A750	100	63.3	37	63	0	0	0
	27	Wt (FN)	del E746–A750	5	3.33	98	2	0	0	0
L858R specific antibody	32	L858R	L858R or Wt	370	350	0	0	0	50	50
	68	L858R	L858R	360	256.7	0	0	63	17	20
	1	L858R	L858R	240	138.3	10	62	8	20	0
	66	L858R	L858R	240	230	0	0	70	30	0
	51	L858R	L858R or Wt	230	173.3	0	37	53	10	0
	31	L858R	L858R or Wt	200	133.3	0	67	33	0	0
	12	L858R	L858R or Wt	100	66.7	33	67	0	0	0
	21	L858R	L858R	80	36.7	63	37	0	0	0
	60	L858R	L858R	20	16.7	83	17	0	0	0
	65	L858R (FP)	Wt	215	138.3	0	0	94	3	3
	58	L585R (FP)	Wt	180	146.7	0	55	43	2	0

^*other deletions; L858R = 2573 T>G, and 2573 T>K (K = G or T)