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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Cryobiology. 2010 Jul 3;61(2):231–233. doi: 10.1016/j.cryobiol.2010.06.011

Figure 3.

Figure 3

(Top panel) Thermal cycle of a 0.1 μL aqueous sample (EAFS 10/10 vitrificatrion solution [8]) on a Cryotop ® instrumented with a 50 μm Cu-Constantan thermocouple and rapidly moved from room temperature air to LN2, held 4 sec, and rapidly immersed in a room-temperature water bath. (Bottom panel) Details of events during warming between ~7.8 sec and 8.0 sec when the sample was moved from LN2 to room temperature water The upward sloping straight line was visually fit to the data and yields the warming rate of 96,000 °C/min. The temperature sampling rate is 200 readings per sec.