Figure 1.

Figure 1A. FACS Analysis of Murine and Chick Cultures at Day 9. Both camptothecin and staurosporine induced apoptosis in murine cultures. There were more late apoptotic (double-labeled) cells with camptothecin treatment than staurosporine treatment. Chick cultures treated with camptothecin showed a similar profile for apoptosis. * p<0.05 vs. control cultures

Figure 1B. FACS Analysis of Murine Cultures at Day 12. At day 12 (5 days after start of apoptogen treatment) only murine cultures were analyzed. There were more cells undergoing apoptosis with staurosporine treatment and camptothecin treatment compared to controls. There were also more cells undergoing apoptosis in control cultures on day 12 than day 9, likely due to the start of mineralization. ZVAD-fmk treated cultures showed significantly less apoptosis than control cultures. There were significantly more dead cells with apoptogen treatment than control cells, and ZVAD treatment of the murine cultures resulted in a lower amount of dead cells than all other conditions, including control cultures. There were no differences in the amounts of late apoptotic cells by day 12. * p<0.05 vs. control cultures