. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: Inflamm Bowel Dis. 2010 Oct;16(10):1729–1738. doi: 10.1002/ibd.21267

TABLE 2.

Primers Used for Quantitative Real-time PCR

Name	Direction	Primer (5′-3′)
Universal qPCR primer	Reverse	NCode miRNA First-strand cDNA Synthesis Kit (Invitrogen)
Let-7d	Forward	AGAGGTAGTAGGTTGCATAGT
Let-7i	Forward	TGAGGTAGTAGTTTGTGCTGT
miR-22	Forward	AAGCTGCCAGTTGAAGAACTGT
miR-106a	Forward	AAAAGTGCTTACAGTGCAGGTAGC
miR-107	Forward	AGCAGCATTGTACAGGGCTATCA
miR-126	Forward	TCGTACCGTGAGTAATAATGC
miR-16	Forward	TAGCAGCACGTAAATATTGGCG
miR-191	Forward	CAACGGAATCCCAAAAGCAGCT
miR-19b	Forward	TGTGCAAATCCATGCAAAACTGA
miR-200c	Forward	TAATACTGCCGGGTAATGATGG
miR-20a	Forward	TAAAGTGCTTATAGTGCAGGTAG
miR-21	Forward	TAGCTTATCAGACTGATGTTGA
miR-215	Forward	ATGACCTATGAATTGACAGAC
miR-223	Forward	TGTCAGTTTGTCAAATACCCC
miR-23a	Forward	ATCACATTGCCAGGGATTTCC
miR-23b	Forward	ATCACATTGCCAGGGATTACC
miR-26a	Forward	TTCAAGTAATCCAGGATAGGC
miR-29a	Forward	TAGCACCATCTGAAATCGGTT
miR-31	Forward	GCAAGATGCTGGCATAGCTG
miR-320	Forward	AAAAGCTGGGTTGAGAGGGCGAA
miR-422b	Forward	CTGGACTTGGAGTCAGAAGGCC
miR-594	Forward	ATCTGGGGTGGCCTGTGACTTT
miR-629	Forward	GTTCTCCCAACGTAAGCCCAGC
U6B	Forward	CGCAAGGATGACACGCAAATTCG