Fig. 2.

Pax2 is not sufficient to confer otic identify to ectodermal cells. Pax2-GFP (B, B′, D, D′, green; E, brown) or GFP (A, A′, C, C′, green) was misexpressed at the 0–1 somite stage. While Gata3 expression is induced ectopically (D′, d, d′, arrow head), Eya1 (B′, b′) and Pax2 (E, e′) are not. Pax2 misexpression in the otic placode leads to loss of Eya1 (B′, b; arrow head) and Pax2 (E, e, arrow head), but expression of Gata3 does not change (d). No effect is observed in control electroporated embryos (A′, a, a′, C′, c). Black lines in A′–E indicate the level of sections shown in a–e and a′–e′.