Fig. 4.

Pax2 controls cell shape in the otic placode. A. Otic precursors were electroporated with control MOs or GFP (top), Pax2 MOs (middle) or Pax2-GFP (bottom). While control cells have elongated at the 13–15 somite stage, Pax2 loss and Pax2 overexpression (Pax2 OE) result in loss of placode cell morphology. Right: five representative cells for each condition. B. Compared to control electroporated cells (WT 13–15ss) the EI is significantly reduced (***) in cells electroporated with Pax2 MOs or Pax2-GFP (Pax2 OE). For comparison measurements from 6 somite placodes are included (WT 6ss). Graph shows mean values ± standard deviation.