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. 2010 Sep 15;345(2-3):180–190. doi: 10.1016/j.ydbio.2010.07.007

Fig. 5.

Fig. 5

Pax2 is required for N-cadherin and N-CAM expression. A. Control electroporated otic placodes (top row) express N-cadherin (magenta) at the 15–16 somite stage. Loss of Pax2 (Pax2 MO, middle row) leads to loss of N-cadherin (open arrow heads, magenta), while N-cadherin is upregulated (white arrow heads) when Pax2 is misexpressed (Pax2 OE, bottom row). Note: N-cadherin is localised apically. B. Control electroporated otic placodes express N-CAM at the 12–13 somite stage (top row, magenta). Loss of Pax2 (Pax2 MO, middle row) leads to loss of N-CAM (open arrow heads, magenta). In contrast overexpression (Pax2 OE, bottom row) results in increased N-CAM (white arrow heads). Note: N-CAM is localised along the entire cell surface. C. N-CAM is not expressed in trunk ectoderm (control), however ectopic expression of Pax2 (Pax2 OE) in this tissue leads to upregulation of N-CAM in electroporated cells (green, white arrow heads), but not in non-electroporated neighbours (open arrow heads); *indicates somite.