Fig. 7.

Sox2 is not sufficient to rescue cell shape or placode invagination in the absence of Pax2.

Otic precursors were electroporated with Pax2 MOs and Sox2 at HH6/7. Cell shape and invagination of the otic placode remain disturbed: compare the non-electroporated control side (left) and the Pax2 MO/Sox2 expressing contralateral side (green, open arrow head or arrow). Pax2 expression (top row, magenta, open arrow head) is absent in electroporated cells; Sox2 does not rescue N-cadherin expression (bottom row, magenta, open arrow). Panels on the right show higher magnification of the targeted area.