Figure 6.

Identification of plasminogen activators and proteases in 11% polyacrylamide gel containing gelatin. The gel contains 11% polyacrylamide, 0.1% SDS, 0.1% copolymerized gelatin and 13 μg/ml plasminogen. Samples were electrophoresed at a constant current of 8 mA in 4°C temperature as follows: Lane3 Reference protein mixture lane 1: Actylase (full length t-PA), lane 2: Actylase +polyclonal anti-t-PA rabbit antibody, lane 4: negative control, lane 6: transfected CHO cells supernatant, lane 5: transfected CHO cells supernatant+anti-t-PA antibody. After electrophoresis the gels were washed in Triton X-100, incubated in glycine-NaOH buffer at 37°C for 3 h and fixed, stained, and destained as described under Method.