Figure 3.

Expression of PRIC295 in human and mouse tissues. (a) Northern blot analysis of PRIC295 mRNA expression using a human multiple tissue blot (Clontech). Blot contains 2 μg of polyA RNA in each lane from tissues indicated. PRIC295 transcript (∼8.5 kb) is expressed in nearly all tissues. (b) Quantitative PCR data showing the expression of PRIC295 mRNA in several different mouse tissues. Primers were designed to amplify from the exon 27-28 region of the cDNA sequence. Samples were normalized with 18S RNA. RNA from 5 different C57B6/J males was pooled and samples were run in triplicate. Standard deviations are shown.