Figure 5.

PRIC295 functions as a transcriptional coactivator for nuclear receptors. Data are shown for PPARα (a), PPARγ (b), and ERα (c). pGL3-3xPPRE-LUC was used as a reporter for PPARα and PPARγ whereas 3xERE was used as reporter for ERα. HeLa cells were transfected with 100 ng of pcDNA-PRIC295, pCMX-PPARα, pCMX-pCMX-PPARγ, pCMX-ERα, pCMX-RXRα, pGL3-3xPPRE-LUC (or empty pGL3-LUC vector), and 50 ng of CMV-RL plasmid as indicated (+ or −). Each column shown is the mean relative luciferase unit value for triplicate experiments. Open bar represents activity in the absence and dark bar in the presence of respective ligand (see Figure 4). All experiments were normalized against the expression of Renilla luciferase (CMV-RL) as an internal control. Statistical significance (P-value) is indicated at the base of the panel and calculated by comparing transfected groups marked with an *. (d) Transcriptional activation of PPARα with the addition of the increasing amounts of PRIC295. All other plasmids were transfected using the same amounts indicated above.