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. 2010 Sep 14;2010:632041. doi: 10.4061/2010/632041

Table 1.

Sequences of TBA oligonucleotide derivatives used in the development of the hAGT fluorescence assay. MeG represents O6-methylguanine. MB represents the fluorophore group (FAM), labelled in the 5′ end, and the quencher group (Dabsyl), labelled in the 3′ end of the sequence.

Abbreviation Sequence
TBA 5′-GGT TGG TGT GGT TGG-3′
5-O6-MeG-TBA 5′-GGT TMe GG TGT GGT TGG-3′
6-O6-MeG-TBA 5′-GGT TG Me GTGT GGT TGG-3′
C-TBA 5′-CCA ACC ACA CCA ACC-3′
MB-TBA 5′-FAM-GGT TGG TGT GGT TGG-Dabsyl-3′
MB-5-O6-MeG-TBA 5′-FAM-GGT T Me GG TGT GGT TGG- Dabsyl-3′
MB-6-O6-MeG-TBA 5′-FAM-GGT TG Me G TGT GGT TGG- Dabsyl-3′
3-HP-TBA 5′-A CCT TTT GGT TGG TGT GGT TGG-3′
6-HP-TBA 5′-C CAA CCT TTT GGT TGG TGT GGT TGG-3′
9-HP-TBA 5′-A CAC CAA CCT TTT GGT TGG TGT GGT TGG-3′