Table 1.
Primers used in PCR amplification. The non-nested primers were selected from those used by Rocho et. al..[15] The original citation is also indicated below.
| Primer | Sequence 5’ to 3’ |
Target | Amplicon size (bp) |
|---|---|---|---|
| Helicobacter genus | |||
| HS-1[9] | AACGATGAAGCTTCTAGCTTGCTAG | 16S rDNA | 400 |
| HS-2 | GTGCTTATTCGTTAGATACCGTCAT | ||
| C97[4] | GCTATGACGGGTATCC | 16S rDNA | 400 |
| C98[4] | GATTTTACCCCTACACCA | ||
| Helicobacter pylori | |||
| HPY S[23] | AGGTTAAGAGGATGCGTCAGTC | 23S rDNA | 267 |
| HPY A[23] | CGCATGATATTCCCATTAGCAGT | ||
| Hemi-Nested PCR | |||
| Helicobacter genus | 163 rDNA | ||
| Outer primers[24] | TATGACGGGTATCCGGC | 375 | |
| HG-1 | ATTCCACCTACCTCTCCCA | ||
| HG-2 | |||
| Inner Primers | CTGAGACACGGTCCAGACTC | 293 | |
| HG-3 | CAAATGCAGTTCTRYRGTTAAGC | ||
| HG-2 | |||
| CTNNB1 | |||
| For | ATGGAACCAGACAGAAAAGC | Beta- catenin |
200 |
| Rev | GCTACTTGTTCTTGAGTGAAG |