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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Nature. 2010 Apr 29;464(7293):1357–1361. doi: 10.1038/nature08938

Fig. 2. Cholesterol crystals activate the NLRP3 inflammasome.

Fig. 2

a, b, Resting or LPS-primed human PBMCs were treated with cholesterol crystals as indicated, MSU crystals (250 µg/ml) or ATP in the presence or absence of the caspase-1 inhibitor zYVAD-fmk (10mM) (b). ELISA and immunoblot (IB) were performed for IL-1β. c, d, IB for caspase-1 in supernatants and cell lysates (c) or ELISA for IL-1β in supernatants (d) from LPS-primed wild-type, NLRP3- or ASC-deficient macrophages stimulated with cholesterol crystals, transfected dsDNA (dAdT), nigericin or ATP. e, IL-18 ELISA of supernatants from LPS-primed wild-type macrophages stimulated with cholesterol crystals or nigericin. Means and s.e.m. of four donors (a, b) or one out of three independent experiments (c, d, e) are shown.