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. 2010 Sep 17;25(10):1418–1426. doi: 10.3346/jkms.2010.25.10.1418

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© 2010 The Korean Academy of Medical Sciences.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4 — Osteogenic differentiation from placenta derived cells. For osteogenic differentiation, first-trimester placenta derived cells were cultured for up to 3 weeks in osteogenic medium (OM). (A) First-trimester placenta derived cells aggregate in 7 days and formed nodules at 14-21 days. (B) First-trimester placenta derived cells exhibit alkaline phosphatase activity at 2 weeks. (C) Von Kossa staining-positive aggregates or nodules are found after 3 weeks, which shows significant calcium deposition in osteogenic media.