Fig. 4.

Blockage of amyloid β-induced apoptosis by preventing cell surface GD3 accumulation and trafficking. (A) TE671 cells were pretreated with methyl-β-cyclodextrin (0.5 mg/mL) for 1 hr and then exposed to Aβ (40 µM) for 4 hr. Localization of intracellular GD3 was evaluated by double immunofluorescence staining technique with FITC-labeled anti-GD3 and Texas Red-labeled anti-caveolin-1 antibodies. (B) Cell surface GD3 levels after 4 hr of Aβ treatment were analyzed by flow cytometry using FITC-labeled anti-GD3 antibody. (C) Apoptotic cell death was quantified after 24 hr of Aβ treatment by flow cytometry analysis using PI. Results are given as the mean±S.D. of four individual experiments. ^*P<0.05.