Figure 1.

(A and C) Amplification in real-time mode for real-time polymerase chain reaction (PCR) and melting curves generated by LightCycler software and differentiation of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii. (B and D) Specificity of the assay was demonstrated by using human DNA and DNA extracted from pure or axenic cultures of several protozoa and pathogenic bacteria known to infect humans, including Entamoeba coli, Salmonella spp., Shigella spp., Vibrio cholerae, Blastocystis hominis, Giardia lamblia, and Cryptosporidium spp. This figure appears in color at www.ajtmh.org.