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Detection limit of real-time polymerase chain reaction (PCR) assay for (A) Escherichia histolytica, (B) Entamoeba dispar, and (C) Entamoeba moshkovskii. Ten-fold serial dilution of DNA consists of 1:10 = 2 ng; 1:100 = 0.2 ng; 1:1000 = 20 pg; 1:10 000 = 2 pg; 1:100 000 = 0.2 pg. This figure appears in color at www.ajtmh.org.
