Table 1.
Enrichment of transcription factor binding elements in the promoters of mTORC1-induced genes.a
Transcription Factor | Binding Motif | Rap-Sensitive Probes (Total)c | p-valued | q-valuee |
---|---|---|---|---|
SREBP | VNNVTCACCCYA | 33 (82) | 2.41E-07 | 1.28E-05 |
c-Myc (HIF1)b | RACCACGTGCTC | 385 (1164) | 4.52E-06 | 1.45E-04 |
HNF4alpha | VTGAACTTTGMMB | 853 (2084) | 2.17E-05 | 4.34E-04 |
ATF4 | CVTGACGYMABG | 18 (30) | 5.57E-04 | 6.36E-03 |
Over-represented transcription factor-binding motifs in all rapamycin-sensitive genes from both Tsc1−/− and Tsc2−/− MEFs identified using a modified version of the MotifADE method (Mootha et al. 2004; Ma et al. 2008). Only these 4 motifs were in enriched in both cell lines with a q-value<0.01. Numbers for the Tsc2 null cells are provided.
The HIF1-binding hypoxia response element (bold) overlaps with this motif.
Numbers are based on 39,000 gene probes analyzed in triplicate with Affymetrix microarrays, comparing vehicle-treated versus rapamycin-treated (24 h) Tsc2−/− MEFs.
Two-tailed Mann-Whitney rank sum p-value.
Multiple testing corrected false discover rate (FDR) q-value.