. Author manuscript; available in PMC: 2011 Sep 15.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jul 29;878(26):2457–2460. doi: 10.1016/j.jchromb.2010.07.016

Table 1.

Menadione (MD) lower limit of quantification (LLOQ)

Lower Limit of Quantification
Dilution Factor	Expected Concentration (pmole MD/mL urine)	Measured Concentration^B (pmole MD/mL urine)	Measured/Expected (%)	SD (%)	CV (%)
Initial
1	N/A	2.37	N/A	0.16	6.8
Dilutions
2	1.19	1.14 ^w	96.2%	0.11	9.39
4	0.59	0.60 ^x	101.3%	0.06	9.36
8	0.30^A	0.30 ^y	101.3%	0.02	6.84
16	0.15	0.16 ^z	108.0%	0.04	31.84
32	0.07	0.09 ^z	121.5%	0.07	71.72

LLOQ of menadione

Concentrations with different superscripts are significantly different using Tukey’s adjusted multiple comparisons of groups’s means (P values <0.05).