Figure 6. Impaired NAE accumulation is observed following FAAH inhibition in brains from mice lacking GDE1 and NAPE-PLD.
Mice were treated with the selective FAAH inhibitor PF-3845 (10 mg/kg, i.p.) and, after 3 hours, sacrificed and brain NAE levels measured. Significant reductions were observed for several NAEs, including the endocannabinoid anandamide (C20:4), in brains from GDE1(−/−)/NAPE-PLD(−/−) mice. The results are presented as means ± standard error. n = 4 mice/group. The symbols above the bars designate p < 0.05, p < 0.01, p < 0.005 (student’s t-test) with *, **, *** for different from wild-type, #, ##, ### for different from GDE1(−/−) samples and †, ††, ††† for different from NAPE-PLD(−/−) samples, respectively.