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. Author manuscript; available in PMC: 2011 Mar 16.
Published in final edited form as: Nature. 2010 Aug 25;467(7313):347–351. doi: 10.1038/nature09323

Figure 4. The (CENP-A/H4)2 heterotetramer assembles with H2A/H2B dimers into an octameric nucleosome with conventional handedness of DNA wrapping.

Figure 4

a, Basic residues that form contacts with nucleosomal DNA are highlighted on (H3/H4)2 (left) and the putative DNA binding surface of (CENP-A/H4)2 (right). b, Model highlighting alterations in nucleosome structure based on the structure of (CENP-A/H4)2. c, Scheme for nucleosome assembly and analysis. d, Histone content of assembled H3- and CENP-A-containing nucleosomes. e, Digestion of nucleosome arrays with micrococcal nuclease reveals that both H3- and CENP-A-containing nucleosomes protect ~150 bp of DNA. f-i, Topological analysis of H3- containing (f and g) and CENP-A-containing (h and i) nucleosomes. Analysis by gel electrophoresis in the absence (f and h) or presence (g and i) of chloroquine reveals that both H3-containing and CENP-A-containing nucleosomes wrap DNA in a left-handed manner.