FIG. 3.

Closed head injury (CHI)–induced alterations of N-methyl-d-aspartate receptor (NMDAR) and AMPA receptor (AMPAR) subunits are localized to the synaptic membranes. (A) At 15 min following CHI, the hippocampus ipsilateral to the injured side was removed, homogenized, and synaptosomal (P2) and light membranes (S2) fractions were isolated. Samples from sham and CHI mice (50 μg/lane) were resolved on 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). Membranes were probed with anti-NR1, anti-NR2A, and anti-NR2B antibodies. Histogram depicts the level of proteins presented as mean ± SD percent of (sham) control (n = 6 pools of 3 animals in each pool). **p < 0.01; *p < 0.05; significantly different from sham (Mann-Whitney test). (B) At 15 min following CHI, the cortex ipsilateral to the injured side was removed, homogenized and total protein were isolated and analyzed as in A. Histogram depicts the level of proteins presented as mean ± SD percent of (sham) control (n = 4 pools of 3 animals in each pool). **p < 0.01; *p < 0.05; significantly different from sham (Mann-Whitney test). (C) Animals were treated as in A, and membranes containing hippocampal proteins were probed with anti-GluR1 and anti-transferrin receptor (TfR) antibodies. Histogram depicts the level of proteins presented as mean ± SD percent of (sham) control (n = 4 pools of 3 animals in each pool). **p < 0.01; significantly different from sham (Mann-Whitney test). (D) Cortical samples were analysed as in C. Histogram depicts the level of proteins, presented as mean ± SD percent of (sham) control (n = 4 pools of 3 animals in each pool). **p < 0.01; significantly different from sham (Mann-Whitney test).