Figure 1.

Systemic administration of LPS induces early gene regulation in the brain, consistent with an inflammatory response. C57BL/6 mice were administered saline or LPS (0.2 mg/kg, i.p.). At varying times (3, 24, and 72 h) after treatment, mice (n = 4 per time point) were killed, and cortical brain tissue was collected. RNA was isolated and hybridized to Affymetrix gene chips (MOE430). A, Graph represents the number of genes differentially regulated in LPS- or saline-treated mice compared with unhandled controls. Time of subsequent stroke is denoted with a black arrow. B, Putative biological functions were assigned to the regulated genes using available public databases and published literature.