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a) Chemical structures of ATP (1) and the γ-phosphate-modified ATP analogues ATP-biotin (2) and ATP-ArN3 (3). b) Kinase-mediated phosphorylation of a hydroxy-containing amino acid (serine is shown) with ATP-ArN3 (3) produces a photo-cross-linking protein product 5. The phosphoramidate bond is cleaved with acid (50% TFA). c) UV irradiation during kinase catalysis promotes covalent cross-linking of the kinase and the substrate through the ATP-ArN3 probe.
