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Schematic of layer-by-layer hepatic tissue formation. Using incremental spacers combined with alternate photomasks allows for the layer-by-layer creation of cell-laden tissues in specific microarchitectural organization (A). Perfusion of micropatterned hepatic tissues demonstrated significant improvements in albumin and urea secretion over unpatterned controls (B, C). Reprinted with permission from Tsang et al.109 Color images available online at www.liebertonline.com/ten.
