Figure 5.

Reconstitution ability of intrafemorally transduced HSC following transplants. Percent donor chimerism (CD45.2-positive cells) in peripheral blood cells from recipients transplanted with total BM from (a) IF-injected WT, FancC ^−/−, or FancA ^−/− mice and (b) from WT or FancC ^−/− mice that received chronic MMC treatments at 7 weeks following IF-injection as a function of time. Each dot represents donor chimerism of an individual recipient. Horizontal line: mean value. (c) Representative cytometric profiles of FancC-EGFP expression in peripheral blood cell lineages from transplanted mice at 3 months following transplantation with BM cells from FancC ^−/− IF-injected animals.