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. 2010 Jul 15;42(9):606–613. doi: 10.3858/emm.2010.42.9.059

Figure 3.

Figure 3

Effects of Smad7 and Smurf1 on TGF-β1-induced, Smad3/4-mediated VEGF transcription. DC2.4 cells (2 × 105/well) were transfected with Smad3/4, Smad7, and Smurf1 (1 µg each). Cells were incubated with CoCl2 (50 µM) and TGF-β1 (1 ng/ml) for 24 h. Total RNA was isolated and levels of endogenous VEGF transcripts were measured by RT-PCR.