Figure 4.

Increases in both the SR Ca²⁺ content and resting cytoplasmic Ca²⁺ level in MDG/TRPC3 KD myoblasts. (A) the SR Ca²⁺ of wild-type or MDG/TRPC3 KD myoblasts loaded with fura-2 was depleted by treatment with 10 µM cyclopiazonic acid (CPA) in the absence of extracellular Ca²⁺. The peak area for 400 sec normalized to that of wild-type controls is shown in the histograms (right). Results are means ± SE (58 wild-type or 55 MDG/TRPC3 KD myoblasts). Each trace is an average of 4 traces. (B) Resting cytoplasmic Ca²⁺ levels of wild-type or MDG/TRPC3 KD myoblasts are shown as histograms. Results are means ± SE (31 wild-type or 34 MDG/TRPC3 KD myoblasts). Both the SR Ca²⁺ content and resting cytoplasmic Ca²⁺ level were increased in MDG/TRPC3 KD myoblasts. ^*Significant difference compared with wild-type (P < 0.05).