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. 2010 Jul 19;42(9):614–627. doi: 10.3858/emm.2010.42.9.061

Figure 6.

Figure 6

Decreases in the expression level of TRPC4, Orai1, and MG29 in MDG/TRPC3 KD myoblasts. Solubilized cell lysate from wild-type or MDG/TRPC3 KD myoblasts (30 µg of total protein) was subjected to SDS-PAGE (10% or 12% gel) and immunoblot assay with anti-TRPC1, anti-TRPC4, anti-TRPC6, anti-STIM1, anti-Orai1, or anti-MG29 antibody. In MDG/TRPC3 KD myoblasts, the expression of TRPC4, Orai1, and MG29 were dramatically reduced. Results are means ± SE of three independent experiments. *Significant difference compared with wild-type (P < 0.05).