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. 2010 Oct;136(4):367–387. doi: 10.1085/jgp.201010430

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© 2010 Bailey et al.

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Figure 13. — Trp²⁸¹ recapitulates the PCMBS-mediated modulation in deactivation kinetics. Activation and deactivation kinetics were estimated with Ca²⁺ jump experiments as described in Fig. 4 using Ca²⁺_i concentrations (A) 0.5, (B) 0.7, (C) 1.0, and (D) 10 µM for L281W (blue trace) and KCa3.1 in the absence (black trace) and presence (red trace) of PCMBS, included for comparison. Activation (E) and deactivation (F) rates were estimated by fitting activation and deactivation records with an exponential function and reported as a time constant (τ-on and τ-off) for KCa3.1 (▴), KCa3.1 PCMBS (▵), L281W (•), L281W PCMBS (○), and V282W (□), see also Table I.