Table II.
Summary of the six-state model used to describe the kinetic behavior of KCa3.1 ± PCMBS
| KCa3.1 | KCa3.1 + PCMBS | Units | |||
| A n = 2 | A n = 1 | B n = 1 | C n = 1 | ||
| 12 | 27 ± 3 | 10 | 27 | 20 | μM−1 s−1 |
| 21 | 34 ± 8 | 76 | 34 | 34 | s−1 |
| 23 | 5425 ± 2725 | 2911 | 5425 | 2820 | μM−1 s−1 |
| 32 | 190 ± 40 | 2978 | 190 | 190 | s−1 |
| 34 | - | - | - | - | - |
| 43 | - | - | - | - | - |
| 35 | 34 ± 7 | 291 | 34 | 34 | s−1 |
| 53 | 20 ± 5 | 3 | 3 | 3 | s−1 |
| 46 | - | - | - | - | - |
| 64 | - | - | - | - | - |
| error | 38 | 45 | 42 | 43 | |
| KCa3.1 L281W | KCa3.1 L281W | ||||
| A n = 1 | B n = 1 | ||||
| 12 | 11 | 27 | μM−1 s−1 | ||
| 21 | 76 | 34 | s−1 | ||
| 23 | 12380 | 5425 | μM−1 s−1 | ||
| 32 | 192 | 190 | s−1 | ||
| 34 | - | - | - | ||
| 43 | - | - | - | ||
| 35 | 53 | 34 | s−1 | ||
| 53 | 5 | 4 | s−1 | ||
| 46 | - | - | - | ||
| 64 | - | - | - | ||
| error | 8 | 13 |
Rate constants derived from fitting the model to KCa3.1 activation and deactivation records. A, rates constants derived using the model with all free parameters: KCa3.1, KCa3.1+PCMBS, and KCa3.1 L281W; B, rates constants derived using the k53 model: KCa3.1+PCMBS and KCa3.1 L281W; C, rates constants derived using the k12 k23 and k53 model: KCa3.1+PCMBS. Bold, fixed to kKCa3.1(-PCMBS).