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. 2010 Oct;136(4):483–494. doi: 10.1085/jgp.200910376

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© 2010 Corry et al.

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Figure 1. — Confocal fluorescence images of AF488- and AF568-labeled MscL protein reconstituted into liposomes. Images of the donor (AF488) are shown before (left column) and after (middle column) acceptor photobleaching. Images are colored by intensity according to the top scale bar. An image created from pixel by pixel analysis of the FRET efficiency is shown in the right column, colored according to the bottom scale bar. The mutant name and conditions are noted on the left, and the average FRET efficiency for the image is noted on the right. I, inner liposome; O, outer liposome.