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. 2010 Oct;136(4):483–494. doi: 10.1085/jgp.200910376

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© 2010 Corry et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 4. — FRET efficiency versus LPC concentration. (A) For mutant M12C, the measured FRET efficiency shows no change upon the addition of LPC. (B) For mutants A27C, V33C, and L86C, the FRET efficiency decreases upon the addition of LPC, but it does not decrease further when LPC concentration is >6 µM. The average post-LPC FRET values and associated uncertainties (as reported in Table II) are represented by the solid and dashed lines on the right-hand side of the figure.