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. 2010 Oct;136(4):443–454. doi: 10.1085/jgp.201010477

Figure 2.

Figure 2.

Gαo2-mediated light responses measured in Gαo1−/− Cx36−/− AIIACs. (A and B) Flash response families were recorded in a Cx36−/− (i.e., Gαo1+/+ Cx36−/− littermate) AIIAC (A) and a Gαo1−/− Cx36−/− AIIAC (B). Flash strengths in the Cx36−/− AIIAC were 0.04, 0.1, 0.22, 0.46, 0.94, 1.9, and 3.8 Rh*/rod, and in the Gαo1+/+ Cx36−/− AIIAC were 0.63, 1.5, 3.1, 6.5, 13, and 27 Rh*/rod. (C) Normalized response amplitudes from individual families were averaged across cells for Cx36−/− AIIACs (n = 10) and Gαo1−/− Cx36−/− AIIACs (n = 9), and plotted as a function of the flash strength. Half-maximal flash strengths estimated from the Hill curve fits were 0.17 ± 0.01 and 2.56 ± 0.13 Rh*/rod (mean ± SEM) for Cx36−/− and Gαo1−/− Cx36−/− AIIACs, respectively. (D) Changes in the amplitude of the maximal flash response as a function of time before, during, and after application of APB are plotted. (E) Maximal flash responses (27 Rh*/rod) in a Gαo1−/− Cx36−/− AIIAC before, during, and after the bath application of 8 µM APB, as marked by upward arrows in D.