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. 2010 Sep 27;207(10):2225–2237. doi: 10.1084/jem.20092712

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© 2010 Guo et al.

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Figure 4. — Chromatin binding assays for monoclonal antibodies and engineered revertants lacking somatic mutations. Standard errors are shown. R and red font color denote germline revertants. Two of the original monoclonal antibodies (J3.4 and J9.11) demonstrated binding activity against several chromatin components as shown in B–D. All antibodies were purified by a stringent affinity method designed to remove potentially contaminating nuclear antigens (Guth et al., 2003). A prototypical ANA, 3H9/Vκ4, is shown in blue in A. Bound antibodies were detected in a solid-phase europium (Eu³⁺)-based fluoroimmunometric assay (as counts per second). One of three experiments with similar results is shown.