Table I.
Summary of hybridoma V region genes
| Hybridoma | Vκ germline genes and shared mutations; shared replacementsa | Mutations: total; replacements | VH germline genes, isotype, and shared mutations; shared replacements | Mutations: total; replacements | Unanimously shared Arg mutations |
| Lineage #1 (mouse 1) | |||||
| J1.1 | Vκ CR-1/Jκ2 unanimously shared mutation10; 8r | 15; 9r | VH7183.9.15/ DFL16.1/ JH1 γ2b unanimously shared mutations 7r | 11; 10r | 0 Vκ; 2 VH |
| J1.4 | 13; 9r | 13; 11r | 0 Vκ; 2 VH | ||
| J2.6 | 14; 9r | 9; 8r | 0 Vκ; 2 VH | ||
| J2.7 | 14; 10r | 10; 8r | 0 Vκ; 2 VH | ||
| J3.1 | 12; 8r | 8; 7r | 0 Vκ; 2 VH | ||
| J3.4 | 10; 8r | 13; 9r | 0 Vκ; 2 VH | ||
| J3.8 | 11; 9r | 11; 8r | 0 Vκ; 2 VH | ||
| J4.2 | 10; 8r | 11; 9r | 0 Vκ; 2 VH | ||
| J5.8 | 14; 9r | 9; 8r | 0 Vκ; 2 VH | ||
| K5.14 | 14; 9r | 9; 8r | 0 Vκ; 2 VH | ||
| J7.18 | 14; 8r | 13; 9r | 0 Vκ; 2 VH | ||
| J9.7 | 13; 8r | 12; 10r | 0 Vκ; 2 VH | ||
| J10.7 | 10; 8r | 13; 9r | 0 Vκ; 2 VH | ||
| J10.13 | 11; 8r | 11; 7r | 0 Vκ; 2 VH | ||
| K6.23 | 13; 9r | 7; 7r | 0 Vκ; 2 VH | ||
| Lineage #2 (mouse 1) | |||||
| J2.8b | Vκ ai4/ Jκ1 unanimously shared mutations 1r | 2; 2r | VH3609.12.174/ DSP2.2/JH1 γ2c/γ2b unanimously shared mutations 2; 1r | 2; 1r | 0 Vκ; 0 VH |
| J7.13b | 1; 1r | 5; 2r | 0 Vκ; 0 VH | ||
| Lineage #3 (mouse 1) | |||||
| J3.7 | Vκ CR-1/Jκ1 unanimously shared mutations 3; 2r | 19; 12r | VHJ558.59.155/DSP2.2/JH3 γ1 unanimously shared mutations 3; 2r | 21; 12r | 0 Vκ; 1 VHc |
| K6.18 | 11; 7r | 11; 9r | 0 Vκ; 1 VH | ||
| Lineage #4 (mouse 1) | |||||
| J5.5 | Vκ 23-43 /Jκ5 unanimously shared mutations 1r | 5; 4r | VHQ52.2.4/DST4/ JH2 γ2c Unanimously shared mutations 6; 3r | 10; 5r | 1 Vκ; 0 VH |
| J6.1 | 4; 3r | 9; 6r | 1 Vκ; 0 VH | ||
| Lineage #5 (mouse 1) | |||||
| J3.9 | Vκ ai4/ Jκ2 unanimously shared mutations 6r | 9; 8r | VHJ558.26.116/DQ52/JH3 γ1 unanimously shared mutations 5r | 8; 6r | 3 Vκ; 1 VH |
| J3.10 | 7; 7r | 15; 9r | 3 Vκ; 1 VH | ||
| Single clones (mouse 1) | |||||
| J3.11 | Vκ ai4/ Jκ5 | 11; 7r | VHJ558.26.116/DSP2.5/ JH2 γ2c | 25; 15r | 1 Vκ; 0 VH |
| J3.12 | Vκ aa4/ Jκ1 | 2; 0r | VH7183.20.37/DSP2.x/JH4 γ2c | 13; 11r | 0 Vκ; 1 VH |
| J6.2 | Vκ 23-39/Jκ2 | 17; 9r | VHJ558.79.184/DST4/JH4 γ2c | 16; 9r | 1 Vκ; 1 VHd |
| J9.11 | Vκ 23-45/Jκ4 | 1; 1r | VHJ558.75.177/DSP2x/JH1 γ2c | 1; 1r | 1 Vκ; 0 VH |
| J10.12 | Vκ19-25/Jκ1 | 7; 5r | VH3609.12.174/ DSP2.x/JH1 γ2c | 4; 4r | 0 Vκ; 1 VH |
| J10.24 | Vκ ai4/Jκ2 | 8; 4r | VHJ558.26.116/DSP2.2/JH2 γ1 | 3; 3r | 1 Vκ; 0 VH |
| K6.16 | Vκ 23-43/Jκ2 | 10; 6r | VHJ588.2.88/ DFL16.1/JH2 γ1 | 18; 11r | 1 Vκ; 1 VH |
| Lineage #6 (mouse 2) | |||||
| N3.18 | Ai4/Jk2 | 7; 4r | VHJ558.26.116/DSP2.5/JH4 γ2c | 15;13r | 1 Vκ; 0 VH |
| N12.6 | Unanimously shared mutations 3; 1r | 13; 6r | Unanimously shared mutations 14; 12r | 15;13r | 1 Vκ; 0 VH |
| Single clones (mouse 2) | |||||
| Q10.4 | 23-43/Jk5 | 4; 3r | VHJ558.26.116/DFL16.1/JH1 γ2c | 2; 1r | 1 Vκ; 0 VH |
Empty cells in the second and fourth columns indicate the same information as the cell above.
a
Amino acid replacements due to somatic mutation.
b
J2.8 is γ2c; J7.13 is γ2b.
c
Members of this clone share an Arg replacement in VH CDR1 but as a result of different base substitutions (mutations).
d
Arg codon may be the result of either a somatic mutation in CDR3 or a P-element addition.