Figure 5.

Dendritic cells are necessary and sufficient for Th2 responses to HDM allergen. (a) Number of inflammatory cells in the BAL and (b) cytokine production by LN cells of WT or CD11c-DTR animals sensitized and challenged with HDM and treated with DT on day 0. (c) Number of inflammatory cells in the BAL, and (d) Cytokine production by LN cells of animals injected on day 0 i.p with basophils, FcεRI⁺DX5⁻ cells or MHCII⁺CD11c⁺ DCs sorted from the lung draining LNs 1 d after HDM administration. Mice were then challenged with HDM on days 7–11. (e) Immunofluorescence staining of Siglec F⁺ eosinophils (red) in the lungs. DAPI (blue) was used to counterstain nuclei. Data are representative of at least two independent experiments from four to six mice/group. Error bars represent the SEM. *, P < 0.05. Bars, 100 μm.