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. 2010 Sep 27;207(10):2175–2186. doi: 10.1084/jem.20100637

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© 2010 Fourcade et al.

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Figure 1. — Tim-3 is up-regulated and coexpressed with PD-1 on NY-ESO-1–specific CD8⁺ T cells. (A) Representative dot plots from melanoma patients (MP) showing ex vivo Tim-3 expression on A2/NY-ESO-1 157–165, A2/EBV BMLF-1 280–288, A2/CMV 495–503, and A2/Flu-M 58–66 tet⁺ CD8⁺ T cells. As shown for one melanoma patient (MP1), CD8⁺ T cells stained with A2/HIVpol 476–484 tetramers or PE-labeled IgG control Ab were used to establish the threshold for identifying tet⁺ cells and Tim-3⁺ cells, respectively. (B and C) Pooled data showing the percentage (%) and MFI of Tim-3 expression on NY-ESO-1–, CMV-, EBV-, and Flu-specific CD8⁺ T cells, as well as total effector (CD45RA⁺ CCR7⁻) and effector/memory (CD45RO⁺ CCR7⁻) CD8⁺ T cells from nine melanoma patients (B) and nine healthy donors (C). (D) Dot plots from one representative melanoma patient showing ex vivo Tim-3 and PD-1 expression on A2/NY-ESO-1 157–165, A2/EBV BMLF-1 280–288, A2/CMV 495–503 and A2/Flu-M 58–66 tet⁺ CD8⁺ T cells. As control, A2/NY-ESO-1 157–165 tet⁺ CD8⁺ T cells stained with PE-labeled and FITC-labeled IgG control antibodies are shown. (E) Pooled data showing the distribution of NY-ESO-1–, CMV-, EBV-, and Flu-specific CD8⁺ T cells, as well as total tet⁻ CD8⁺ T cells according to Tim-3 and PD-1 expression. Horizontal bars depict the mean percentage or MFI of Tim-3 and/or PD-1 expression on tet⁺ CD8⁺ T cells. The p- values were calculated using the Wilcoxon signed rank test. Data shown are representative of at least three independent experiments.