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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2010 Sep;CHAPTER:Unit–1F.10. doi: 10.1002/9780470151808.sc01f10s14

Figure 1.

Figure 1

Figure 1

Figure 1

Embryoid body formation from hanging droplet. (a) Pipetting 11µL droplets of cells onto the bottom of a 15cm petri dish. (b) The dish is inverted to generate hanging droplets. The droplet density shown here (~250 droplets/plate) yields the maximum number of EBs per plate. (c) A representative area of beating eGFP+ cells within an EB.