DMEM-High Glucose GIBCO 11965-0692 | 500 mL |
Non-Essential Amino Acids (10mM) GIBCO 11140-050 | 6.25 mL |
L-Glutamine0^ (200mM) GIBCO 25030-081 | 6.25 mL |
Penicillin/Streptomycin (10000U/ml, 10000µg/ml) GIBCO 15070-063 | 12.5 mL |
Fetal Bovine Serum* GEMINI 100–106 | 94 mL |
β-mercaptoethanol SIGMA M6250 | 4.4 µL |
Leukemia Inhibitory Factor (107 units/mL) CHEMICON ESG1107 | 62.5 µL |