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. Author manuscript; available in PMC: 2011 Sep 1.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2010 Sep;CHAPTER:Unit–1F.10. doi: 10.1002/9780470151808.sc01f10s14
IMDM GIBCO 12440-061 500 mL
L-Glutamine^ (200mM) GIBCO 25030-081 6.25 mL
Penicillin/Streptomycin (10000U/ml, 10000µg/ml) GIBCO 15070-063 12.5 mL
Fetal Bovine Serum* GEMINI 100–106 94 mL
Monothioglycerol SIGMA M6145 6.5 µL
Leukemia Inhibitory Factor (107 units/mL) CHEMICON ESG1107 62.5 µL