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. 2010 Sep 22;30(38):12844–12855. doi: 10.1523/JNEUROSCI.2437-10.2010

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Copyright © 2010 the authors 0270-6474/10/3012844-12$15.00/0

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Figure 5. — Localization of TNFR1 in spinal cord of naive mice. A, A negative control for TNFR1 staining when the primary antibody to TNFR1 is not added; B, TNFR1 is expressed throughout the spinal cord gray matter. a, b, View of the slices under the light microscope. C, Double immunofluorescence in spinal dorsal horn shows colocalization of TNFR1 (red) and GFAP, an astrocyte marker (green). D, Double immunofluorescence in spinal dorsal horn shows no colocalization of TNFR1 (red) and NeuN, a neuronal soma marker (green). E, Double immunofluorescence shows colocalization of TNFR1 (red) and MAP2, a neuronal dendrite marker (green). F, Double immunofluorescence shows no colocalization of TNFR1 (red) and CD11b, a microglia maker (green). Scale bars, 5 μm.