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Journal of Bacteriology logoLink to Journal of Bacteriology
. 1980 Nov;144(2):661–665. doi: 10.1128/jb.144.2.661-665.1980

Vacuoles: main compartments of potassium, magnesium, and phosphate ions in Saccharomyces carlsbergenis cells.

L A Okorokov, L P Lichko, I S Kulaev
PMCID: PMC294715  PMID: 7430066

Abstract

The uneven distribution of Mg2+, K+, and phosphate in Saccharomyces carlsbergensis was demonstrated by the differential extraction of ions. Their concentrations were 5, 60, and 1 mM in the cytoplasm and 73, 470, and 110 mM in vacuoles, respectively. The intracellular gradients of these ions were 1:15, 1:8, and 1:110, respectively, across the tonoplast. The determination of free Mg2+ (1.35 mM in the cytosol and 20 mM in vacuoles) showed that the ion accumulation in vacuoles could not be explained by the higher degree of ion complexing in these organelles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAUM P., CZOK R. [Enzymatic determination of "ionized" magnesium in plasma]. Biochem Z. 1959;332:121–130. [PubMed] [Google Scholar]
  2. Boller T., Dürr M., Wiemken A. Characterization of a specific transport system for arginine in isolated yeast vacuoles. Eur J Biochem. 1975 May;54(1):81–91. doi: 10.1111/j.1432-1033.1975.tb04116.x. [DOI] [PubMed] [Google Scholar]
  3. Delhez J., Dufour J. P., Thines D., Goffeau A. Comparison of the properties of plasma membrane-bound and mitochondria-bound ATPases in the yeast Schizosaccharmoyces pombe. Eur J Biochem. 1977 Sep 15;79(1):319–328. doi: 10.1111/j.1432-1033.1977.tb11812.x. [DOI] [PubMed] [Google Scholar]
  4. Günther T. Die intrazelluläre Mg-Ionenkonzentration. Z Naturforsch B. 1967 Feb;22(2):149–154. [PubMed] [Google Scholar]
  5. Günther T., Dorn F. Die intracelluläre Mg-Ionenaktivität in verschiedenen Säugetierzellen. Z Naturforsch B. 1971 Feb;26(2):175–177. [PubMed] [Google Scholar]
  6. Lin W., Wagner G. J., Siegelman H. W., Hind G. Membrane-bound ATPase of intact vacuoles and tonoplasts isolated from mature plant tissue. Biochim Biophys Acta. 1977 Feb 14;465(1):110–117. doi: 10.1016/0005-2736(77)90359-5. [DOI] [PubMed] [Google Scholar]
  7. Lusk J. E., Williams R. J., Kennedy E. P. Magnesium and the growth of Escherichia coli. J Biol Chem. 1968 May 25;243(10):2618–2624. [PubMed] [Google Scholar]
  8. Okorokov L. A., Kadomtseva V. M., Titovskii B. I. Transport of manganese into Saccharomyces cerevisiae. Folia Microbiol (Praha) 1979;24(3):240–246. doi: 10.1007/BF02926455. [DOI] [PubMed] [Google Scholar]
  9. Okorokov L. A., Lichko L. P., Kholodenko V. P., Kadomtseva V. M., Petrikevich S. B., Zaichkin E. I., Karimova A. M. Free and bound magnesium in fungi and yeasts. Folia Microbiol (Praha) 1975;20(6):460–466. doi: 10.1007/BF02891704. [DOI] [PubMed] [Google Scholar]
  10. Ramos F., Thuriaux P., Wiame J. M., Bechet J. The participation of ornithine and citrulline in the regulation of arginine metabolism in Saccharomyces cerevisiae. Eur J Biochem. 1970 Jan;12(1):40–47. doi: 10.1111/j.1432-1033.1970.tb00818.x. [DOI] [PubMed] [Google Scholar]
  11. Roomans G. M., Sevéus L. A. Subcellular localization of diffusible ions in the yeast Saccharomyces cerevisiae: quantitative microprobe analysis of thin freeze-dried sections. J Cell Sci. 1976 Jun;21(1):119–127. doi: 10.1242/jcs.21.1.119. [DOI] [PubMed] [Google Scholar]
  12. Schmidt G. B., Rosano C. L., Hurwitz C. Evidence for a magnesium pump in Bacillus cereus T. J Bacteriol. 1971 Jan;105(1):150–155. doi: 10.1128/jb.105.1.150-155.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Veloso D., Guynn R. W., Oskarsson M., Veech R. L. The concentrations of free and bound magnesium in rat tissues. Relative constancy of free Mg 2+ concentrations. J Biol Chem. 1973 Jul 10;248(13):4811–4819. [PubMed] [Google Scholar]
  14. Wiemken A., Dürr M. Characterization of amino acid pools in the vacuolar compartment of Saccharomyces cerevisiae. Arch Microbiol. 1974;101(1):45–57. doi: 10.1007/BF00455924. [DOI] [PubMed] [Google Scholar]

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