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. 2010 Aug 17;22(8):2680–2696. doi: 10.1105/tpc.110.075549

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Figure 10. — GA-Dependent Interaction between GID2 and SLR1 in Vitro and in Vivo.

(A) In vitro pull-down assay showing GA-dependent interaction between GST-SLR1 and T7-tagged GID1 and between GST-SLR1 and HA tagged-GID2. GST-SLR1, T7 tagged-GID1, and HA tagged-GID2 were expressed together in E. coli and purified with glutathione beads. E. coli expressing GST-SLR1 and T7-tagged GID1 was used as a control. HA-tagged GID2 was detected with anti-HA antibody.

(B) BiFC analysis of in vivo interaction between GID2 and SLR1 in N. benthamiana leaf epidermis (Abe et al., 2005). BF, bright-field image; EYFP, EYFP fluorescence; DAPI, 4',6-diamidino-2-phenylindole; NY-GID2 CY-SLR1, expression of N•EYFP-GID2 and C•EYFP-SLR1 without GID1; NY-GID2 CY-SLR1+GID1, expression of N•EYFP-GID2 and C•EYFP-SLR1 with nontagged GID1. Leaves were sprayed with 10⁻⁴ M GA₄ dissolved in ethanol (+) or with ethanol alone (–) 10 min before observation of the signals. Bar = 10 μm.