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. 2010 Aug 17;22(8):2680–2696. doi: 10.1105/tpc.110.075549

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© 2010 American Society of Plant Biologists

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Figure 3. — Physicochemical Analysis of the Interaction between GID1 and SLR1^WT, SLR1^G576V, and SLR1 (E4-R125).

(A) Various kinetic constants of GID1-SLR1, GID1-SLR1^G576V, and GID1-SLR1 (E4-R125) interactions in the presence of GA₄. Parameters were calculated by the heterogenous ligand model for three interactions or by the 1:1 binding model for GID1-SLR1^G576V and GID1-SLR1 (E4-R125) interactions. GST-fused SLR1 and SLR1 mutant proteins were used for the analysis.

(B) to (D) Interactions between GID1 and SLR1 proteins in the presence of GA₄. Note that the response unit (RU) value of the GID1-SLR1^WT interaction does not return to the baseline level at the end of the reaction due to a delayed dissociation rate (B) compared with GID1-SLR1^G576V (C) and GID1-SLR1 (E4-R125) (D), as shown by arrows.

(B) GID1-SLR1^WT interaction.

(C) GID1-SLR1^G576V interaction.

(D) GID1-SLR1 (E4-R125) interaction.