Figure 6.

Interaction of GID2^L76A, SLR1, and GID1 in Yeast Cells.

Interactions of BD and AD fusion proteins with or without 10⁻⁴ M GA₄ were scored by β-Gal activity (means ± sd; n = 3). For the Y3H assay, GID2^L76A was used as bait, SLR1 (wild-type or mutant) was used as prey, and either GID1 or gid1-1 mutant protein was expressed as a third clone. The GA-dependent interaction of SLR1 and GID1 proteins is indicated with a circle (strong interaction) or triangle (weak interaction), and “×” indicates no interaction.