Figure 7.

Ala Scanning Analysis of SLR1 for GA-Dependent GID2- and GID1-Interacting Activities.

Interacting activities between 34 mutated SLR1s and GID1 or GID2^L76A are shown by blue and red bars, respectively (means ± sd; n = 3). SLR1^WT and SLR1 (E4-R125) were used as controls (far left). Activities are shown as a relative rate, with activities of wild-type SLR1 set as 1. To measure interactions between SLR1s and GID1, a Y2H assay was performed using GID1 as bait and the mutated SLR1 as prey in the presence of 10⁻⁴ M GA₄. For interactions between SLR1s and GID2, a Y3H assay was performed using GID2^L76A as bait and the mutated SLR1 as prey in the presence of GID1 and 10⁻⁴ M GA₄. The structure of the SLR1 GRAS domain is shown at the bottom of the figure, and regions important for SLR1 to interact with GID1 or GID2 are shown in rectangles at the top. SLR1 mutants, which were analyzed with SPR (Table 1), are marked with black circles.