Table 1.
Various Kinetic Constants of the Interaction between GID1 and mSLR1s Using SPR
| Binding 1 |
Binding 2 |
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| Analyte | Ligand | ka (M−1 s−1) | kd (s−1) | KD (M) (kd/ka) | ka (M−1 s−1) | kd (s−1) | KD (M) (kd/ka) |
| GID1 | SLR1WT | 2.75 × 105 | 1.62 × 10−3 | 5.89 × 10−9 | 8.11 × 105 | 6.48 × 10−2 | 7.99 × 10−8 |
| GID1 | HFY315-7AAA | 2.43 × 105 | 1.30 × 10−3 | 5.36 × 10−9 | 5.19 × 105 | 4.67 × 10−2 | 9.00 × 10−8 |
| GID1 | PLY321-3AAA | 2.43 × 105 | 1.13 × 10−3 | 4.65 × 10−9 | 5.22 × 105 | 5.15 × 10−2 | 9.87 × 10−8 |
| GID1 | HFT327-9AAA | 2.35 × 105 | 1.95 × 10−3 | 8.29 × 10−9 | 5.71 × 105 | 5.39 × 10−2 | 9.44 × 10−8 |
| GID1 | QWP356-8AAA | 1.79 × 105 | 9.64 × 10−4 | 5.39 × 10−9 | 6.90 × 105 | 6.23 × 10−2 | 9.03 × 10−8 |
| GID1 | HRL450-2AAA | 1.31 × 105 | 1.06 × 10−3 | 8.09 × 10−9 | 6.50 × 105 | 6.09 × 10−2 | 9.37 × 10−8 |
| GID1 | SLR1 (E4-R125) | 3.99 × 105 | 1.31 × 10−2 | 3.28 × 10−8 | 7.05 × 105 | 5.97 × 10−2 | 8.47 × 10−8 |
| GID1 | SLR1G576V | 2.78 × 105 | 1.38 × 10−2 | 4.96 × 10−8 | 6.03 × 105 | 4.38 × 10−2 | 7.26 × 10−8 |
| GID1 | LQ361-2AA | 1.65 × 105 | 1.48 × 10−2 | 8.97 × 10−8 | 7.29 × 105 | 6.19 × 10−2 | 8.49 × 10−8 |
| GID1 | DRF490-2AAA | 4.35 × 105 | 1.01 × 10−2 | 2.32 × 10−8 | 5.75 × 105 | 5.26 × 10−2 | 9.15 × 10−8 |
| GID1 | HYY497-9AAA | 5.19 × 105 | 7.01 × 10−3 | 1.35 × 10−8 | 5.89 × 105 | 5.44 × 10−2 | 9.24 × 10−8 |